What is crude fat, crude fat in feed First of all, let's understand the concept of crude fat. Crude fat is a general term for fat-soluble substances in feed, animal tissues, and animal excrement. That is, if we want to understand the content of crude fat in the feed, ie soak the feed in ether, the organics in the feed that are soluble in the ether are similar to the real fat, chlorophyll, cholesterol and other substances and are all part of the crude fat content. The "coarse" in crude fat is roughly, roughly meaning, because only fat is soluble in ether, so only a rough estimate can be made. Of course, such an explanation is a bit of meaning in Chinese, but we only need to remember that crude fat contains more than fat! Then, the crude fat content is calculated as follows: crude fat (%) = ether extract weight (g) / feed sample weight (g) * 100%. Method for measuring crude fat in feed Feed is divided into single, mixed, compound feeds and prefeeds. This standard applies to the determination of crude fat in various feeds. The following provides the principle, reagents, instruments, analysis procedures and calculation methods for crude fat in feeds. Feed fat crude fat determination principle and reagent requirements A Soxhlet fat extractor extracts the sample with ether and weighs the extract. Besides fat, it also contains organic acids, phospholipids, fat-soluble vitamins, chlorophyll, etc. The result is called crude fat or ether extract. Reagents: anhydrous ether (Analytical Pure). Feed fat crude fat measuring equipment 1 Laboratory sample grinder or mortar. 2 points sieve: aperture: 0.45mm. 3 Analytical balance: Sensitivity 0.0001g. 4 electric constant temperature water bath: room temperature ~ 100 °C. 5 constant temperature oven: 50 ~ 200 °C. 6 Soxhlet fat extractor (with spherical condenser): 100 or 150 mL. 7 Soxhlet fat extractor. 8 filter paper or filter paper tube: medium speed, degreasing. 9 Dryer: Use calcium chloride (dry grade) or discoloration silica gel as a desiccant. Crude fat measurement analysis step in feed First select the sample: Select a representative sample, use the quarter method to reduce the sample to 500g, crushed to 40 mesh, and then reduced to 200g in a sealed container with four minutes to save. Then use one of the following methods to perform crude fat analysis: 1 Arbitration method: Determined using a Soxhlet fat extractor. The Soxhlet extractor (4.6) should be dry and dry. The extraction flask (with several zeolites inside) was dried in a 105±2°C oven (4.5) for 60 min, cooled in a desiccator (4.9) for 30 min, and weighed. After drying for 30 minutes, the same cooling and weighing, the difference between the two weights is less than 0.0008g is a constant weight. Weigh the sample 1 ~ 5g (accurate to 0.0002g), in the filter paper tube, or wrapped with filter paper, placed in a 105 °C oven (4.5), drying 120min (or into the dry sample after the determination of water, converted into Air-dried sample weight), filter paper tube or bag into the extraction tube, add anhydrous ether (3.1) 60-100 mL in the extraction bottle, heating in a 60-75°C water bath (with distilled water), make ether (3.1) For reflux, control the reflux of ether (3.1) to about 10 times per hour, return 50 times in total (about 70 samples with high oil content) or check the ether (3.1) flowing out of the extraction tube and leave no trace of oil after pumping. To mention the end. Take out the sample, there is still the original extractor to recover ether until the extraction bottle is completely finished, remove the extraction bottle, and evaporate the residual ether on the water bath. Wipe the outer wall of the bottle. Put the extraction bottle into a 105±2°C oven (4.5) to dry it for 120 min, cool it in a desiccator (4.9) for 30 min, and then dry it for 30 min, and then cool and weigh. The difference between the two weights is less than 0.001g. weight. 2 Recommended Method: Determined using a fat extractor (4.7). According to each instrument operating instructions for the determination. Calculation of Crude Fat Measurement Results in Feed 1 Calculation see below Crude fat (%)=(m2-m1)×100/m Where: m - air-dried sample weight, g. M1-- The weight of the extracted bottle, g. M2-- The weight of the extracted bottle with constant weight, g. 2 repeatability Each sample was taken twice in parallel and the arithmetic average was used as the result. When the crude fat content is ≥10%, the allowable relative deviation is 3%. When the crude fat content is 10%, the relative deviation is 5%. By the above method, the content of crude fat in the feed can be obtained. Single-Cylinder Hydraulic Cone Crusher
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