Centrifuge selection guide

How to buy a satisfactory centrifuge? The following will be detailed by Shanghai Bayu technicians for you:

First, according to the sample temperature required to choose:

1. The separation sample can be stored under the normal temperature +15 environment, without changing its characteristics, you can use room temperature centrifuge.

2. If the separation of the sample will be affected by the temperature and change its characteristics, (such as proteins, cells, etc.) will be destroyed in the high temperature environment, which is to choose a refrigerated centrifuge, refrigerated centrifuges have a rated temperature range.

3. Some samples according to the test requirements, need to be heated, (such as oil, milk fat) need to use heating centrifuge.

Second, according to the required centrifugal force to choose:

Centrifuge uses the principle of centrifugal sedimentation to separate, concentrate or purify cells (particles) with different densities in solution. Centrifugal tube with equal amount of test solution is placed symmetrically in the rotor (test tube). After the instrument is started, the motor drives the rotor to rotate at a high speed and the relative centrifugal force (RCF) is generated to separate cells (particles) with different density in the test solution. The magnitude of the centrifugal force depends on the horizontal distance from the position of the specimen to the axis, that is, the rotation radius r and the rotation speed n.

The formula is as follows:

RCF=1.118×10-6n2r×g

n------------ Speed ​​(r/min)

r------------ Rotation radius (mm)

Centrifuge

Third, according to the separation of workload and research needs to choose:

1. Generally use desktop centrifuges for research and small batch preparation;

2. Use large floor-standing centrifuges when making large batches;

3. County-level above the dimethyl level more than laboratory use more with a refrigeration system, desktop high-speed refrigerated centrifuge or floor-type low-speed refrigerated centrifuge, township medical units choose desktop high-speed refrigerated centrifuge;

4. A blood bank medical unit should use a blood centrifuge.

Fourth, according to the brand business choice:

(1) Pay attention to the place of production: The main production and distribution centers for domestic centrifuges are Beijing, Hunan Changsha, Anhui, and Shanghai, among which Hunan Changsha is the most.

(2) Pay attention to the brand: The main production and distribution center will also have many different brands of manufacturers, many manufacturers are keen to show evidence of virtual power: the continuation of the glorious state-owned factory, double high-tech personnel and so on.

(3) Pay attention to modeling: Many manufacturers are actually small-scale assembly plants of 35 shareholders. They have neither R&D capability nor control technology, and their own strength is insufficient. They use a common mold to go through a homogenous production route, resulting in products on the market. Is to bring stereotyped shell shape.

Fifth, the choice of turning

Rotors: Centrifuge rotors are mainly divided into two types.

Horizontal rotor: During the operation, the hanging blue is in a horizontal state and is at right angles to the axis of rotation. The sample concentrates on the bottom of the centrifuge tube.

Angle rotor: The centrifugal vessel is at a fixed angle to the axis of rotation. The sample concentrates the sediment at the bottom of the centrifuge tube and near the bottom wall. If you want the separated sample to concentrate on the bottom of the centrifuge tube, select the horizontal rotor. If you want the sample to concentrate on the bottom of the centrifuge tube and on the side wall near the bottom, select the angular rotor.

There are special tests or special samples that require special rotors such as large-capacity baskets, microplate rotors, slide rotors, PCR rotors, test tube rotors, and capillary rotors. The rotor has a fixed specification, which is combined with the capacity of the centrifuge, such as 36 × 5 ml angle rotor, which determines both the type of rotor and the capacity of the centrifuge, so the choice of rotor is very important.

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